BIOT 643Spring 2017Quiz 3 (75 points)Multiple Choice Questions: Select the one correct answer. (5points each)1) Expression of a protein in an insect cell using the baculovirs method relies upon:A)Creation of a fusion proteinB) Creation of a recombinant virusC) Creation of an artificial chromosomeD)None of the above2) In an SDS-PAGE SDS is a:A) DetergentB) BaseC) AcidD) Electrolyte3) An 2-D protein gel combines an SDS-PAGE gel with:A) Mass spectroscopyB) Charge equilibriumC) Isoelectric focusingD) Mass balance correction4) Which protein detection method is able to measure the distance of molecular interaction byutilizing phenomenology based upon the transfer of energy?A) Far Western analysisB) Phage displayC) Co-immuno-precipitationD) Florescent resonance5) Which is NOT a method used to transfer DNA into a mammalian cell?A)Direct physical introductionB) Lithium acetateC) Viral infectionD)Inorganic salt complex 6) When constructing a protein expression vector for a eukaryotic system which is NOT required?A) A selectable eukaryotic marker geneB) Eukaryotic transcription stop signalsC) A eukaryotic telomere sequenceD) A eukaryotic promotor sequenceA)B)C)D) 7) Which an advantage of a bacterial expression system?Minimally complex growth mediaSlow cell growthSecretion to the mediumProper post-translational folding8) If you are asked to identify the presence of protein X in a mixture of 100 proteins (you havesequence information on that protein and an antibody) which ONE of the following methods willyou use?A)Silver StainingB) Coomassie BlueC) Western BlotD)Real time RT-PCR9) Which array would you chose to monitor cell surface characteristics globally?A)B)C)D) Antibody microarrayReverse-phase protein arrayProteome microarrayLectin microarray Short Answer: Provide specific answers in your own words. 1. Describe the three main steps used when analyzing a molecule (protein) through Ion Mass Spectrometry(10 pts)2. You are a researcher working on human cancer. You have access to the following capabilities in yourlaboratory; co-immunoprecipitation FRET Chromatin Immunoprecipitation or Far Western Blot. Yourteam believes a mutation in a hypothetical growth hormone (overdrive) is associated with ovariancancer. Pick any one of the available capabilities and develop an experiment designed to investigate ifthe overdrive-cancer link is a plausible hypothesis. (20 pts)
