TY PE Y OUR FULL NAME:Biology 102/103Lab 4: EnzymesINSTRUCTIONS:On your own and without assistance complete this Lab4AnswerForm electronicallyand submit it via Assignments by the date listed on your Course Schedule (underContent – Syllabus). NOTE: The Assignment tab closes promptly @ 11:59 PM ETand will not be available even a minute past the deadline. Save yourLab4AnswerForm in the following format: LastName_Lab4 (e.g. Smith_Lab4).I will be posting feedback under a private Lab 4 Feedback group in the Discussionsafter I have graded all the labs. You will gain access to the private Lab 4 area if yousubmit a complete lab on time.You must submit your document in a Word (.doc or .docx) or Rich Text Format (.rtf)to receive credit. I cannot open Works or WordPerfect files.Experiment 1: Enzymes inFood (15 pts)Table 1: Substance vs. StarchPresenceSubstanceResulting ColorPresence of Starch?Positive Control: StarchNegative Control: Student Must SelectFood Product:Food Product:Saliva:Post-Lab Questions1. What were your controls for this experiment? What did they demonstrate? Why was saliva included in this experiment?2. What is the function of amylase? What does amylase do to starch? 3. Which of the foods that you tested contained amylase? Which did not? What experimentalevidence supports your claim?4. Saliva does not contain amylase until babies are two months old. How could this affect aninfantӳ digestive requirements?5. Digestive enzymes in the gut include proteases which digest proteins. Why donӴ theseenzymes digest the stomach and small intestine which are partially composed of protein? Experiment 2: Effect of temperature on enzyme activity (15 pts)Table 2: Record the effect of temperature on the production of gasTubeTemperature(у)BalloonCircumference(Uninflated in cm)Balloon Circumference(Inflated in cm)1 (cold)2 (room temp)3 (hot)Post-Lab Questions1. What is the enzyme in this experiment? What is the substrate?2. What is the independent (IV) and dependent (DV) variables in this study? 3. How does temperature affect enzyme function?4. How can enzyme activity be increased?5. In general how would an increase in substrate alter enzyme activity? Draw orinsert a graph to illustrate this relationship. 6. Draw a graph using balloon diameter vs. temperature. You may use Excel thenԉnsertԠthe graph or use another drawing program. You may also draw it neatlyby hand and scan your drawing. You would need to insert the scanned jpg imagehere in that case. What is the correlation shown on your graph?7. Design an experiment to determine the optimal temperature for enzyme function complete with controls. Be sure to state your hypothesis describe your design dissect your variables (IV/DV) and predict your results. When you describe yourdesign be sure to explain where you will find the enzymes for this experimentand what substrate you will use.
